Search results for " ligand binding"
Article
Alkaline stability of Fc and VH3 binding in protein A resins
In the development of antibody purification protocols, you need to know your Ab molecule and its possible affinity binding sites for the chromatography resins. In this study, we have looked at…
Article
A Closer Look at Affinity Ligands
Release of the protein from the ligand is generally achieved by altering parameters such as pH, and sodium chloride concentration or by the addition of a competitor for ligand binding.
Shamlou (Ab…
Article
Applications of Surface Plasmon Resonance for Detection of Bispecific Antibody Activity
The enzyme-linked immunosorbent assay (ELISA) is often used to assess ligand binding; however, it is an indirect method and the output is limited to end-point data. By contrast, label-free technologie…
Article
Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
SPR is a rapidly emerging tool for studying ligand binding interactions with membrane proteins, which are the major molecular targets for biopharmaceutical products (6). In SPR, one of the interactant…
Article
A Look at the Affinity Chromatography Landscape
A Look at the Affinity Chromatography Landscape
New ligands are being developed to meet the separation and purification needs of next-gen biologics.
By Cynthia A. Challener
…
Article
Re-use of Protein A Resin: Fouling and Economics
Mar 01, 2015
By Anurag S. Rathore, Mili Pathak, Guijun Ma, Daniel G. Bracewell
BioPharm International
Volume 3, Issue 28
In the past two decades, Protein A affinity chromatography ha…
Article
Ligand-Binding Assays and the Determination of Biosimilarity
LBA detection techniques
There are essentially two general methods for assessing ligand binding: ELISA (enzyme-linked immunosorbent assay)-type assays and kinetic binding assays. Classical ELISA a…
Article
Process Chromatography: Continuous Optimization
Improved resin chemistries and customized separation solutions are enabling more efficient separations.
By Cynthia A. Challener
Cell-culture and fermentation are high-…
Article
Enhancing Resins Addresses Purification Concerns
Christoph Burgstedt/Stock.Adobe.com
Enhancement of resin technology can improve the ability of chromatography to purify the growing landscape of increasingly complex biomolecu…
Article
Evolution of the Monoclonal Antibody Purification Platform
The authors discuss the evolution of the purification platform for manufacturing of mAb therapeutics.
Monoclonal antibodies (mAb) increasingly form the majority share of the product pipeline…